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wash buffer  (Thermo Fisher)


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    Structured Review

    Thermo Fisher wash buffer
    Wash Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/wash+buffer/Triton+X-100/pmc13084690-106-8-38
    Average 99 stars, based on 1 article reviews
    wash buffer - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: Mitochondrial control of amino acid catabolism by a fasting-inducible mitochondrial carrier
    Article Snippet: .. The cells were washed three times with PBS and permeabilized with 0.3% NP-40, 0.05% Triton X-100, and 0.1% bovine serum albumin (BSA) in PBS for 3 min. After three rinses with wash buffer (0.05% NP-40, 0.05% Triton X-100, and 0.2% BSA in PBS), the samples were blocked for 1 hour at room temperature with SuperBlock Blocking Buffer (37515, Thermo Fisher Scientific). ..

    Article Title: Scalable synthetic peptide hydrogel enables self-organized luminal cavity architecture within hiPSC 3D colonies supporting functional hepatocyte differentiation
    Article Snippet: PG-hiHs were retrieved and fixed using 10% neutral buffered formalin (NBF) (Sigma). .. Following fixation, the 3D colonies were rinsed with wash buffer (DPBS with Ca 2+ /Mg 2+ supplemented with 0.2% Triton-X 100 and 0.1% gelatin from cold water (all from Sigma‒Aldrich) and subsequently incubated in 10% normal goat serum (Thermo Fisher) in wash buffer overnight for blocking. ..

    Article Title: Protein-state dysregulation and sex-specific neurodevelopmental signatures in schizophrenia forebrain organoids
    Article Snippet: .. After primary antibody incubation, cultures were washed three times in wash buffer (PBS/0.1% Triton X-100) for 15 min at RT and incubated with appropriate fluorophore-conjugated secondary antibodies and nuclei counterstained with DAPI (NucBlue, #R37606, Thermo Fischer Scientific, 1:1000) diluted in blocking buffer, ON at 4°C. ..

    Lysis:

    Article Title: Netrin-1 drives cell-type-specific plasticity of human dopaminergic neurons during circuit integration in a Parkinsonian model
    Article Snippet: Briefly, brain samples were homogenized using a glass dounce homogenizer (24 strokes) in 1.5mL of ice-cold lysis buffer (Nuclei EZ Prep, Sigma, with 0.2U/μl recombinant RNase inhibitor, Takara) and incubated on ice for 5 min with 1.5ml of lysis buffer. .. Centrifuged nuclei (500g, 5 min, 4°C) were washed in 1ml of ice-cold lysis buffer, incubated for 5 min, centrifuged (500g, 5 min, 4°C) and resuspended in 1ml of wash buffer (PBS, Gibco, with 1% Ultrapure Bovine serum albumin, Ambion, and 0.2U/μl RNase inhibitor). .. Isolated nuclei were filtered through a 30μm cell strainer (MACS SmartStrainers), washed with 1ml of wash buffer, centrifuged (500g, 5 min, 4°C), resuspended in 300μl of wash buffer on ice, counted using Trypan blue (ThermoFisher) and stained with primary antibodies, including human nuclear antigen conjugated to Phycoerythrin (HNA-PE, 1:100, Abcam) and rabbit anti neuronal nuclei (rb-NeuN, 1:100, Abcam).

    Incubation:

    Article Title: Netrin-1 drives cell-type-specific plasticity of human dopaminergic neurons during circuit integration in a Parkinsonian model
    Article Snippet: Briefly, brain samples were homogenized using a glass dounce homogenizer (24 strokes) in 1.5mL of ice-cold lysis buffer (Nuclei EZ Prep, Sigma, with 0.2U/μl recombinant RNase inhibitor, Takara) and incubated on ice for 5 min with 1.5ml of lysis buffer. .. Centrifuged nuclei (500g, 5 min, 4°C) were washed in 1ml of ice-cold lysis buffer, incubated for 5 min, centrifuged (500g, 5 min, 4°C) and resuspended in 1ml of wash buffer (PBS, Gibco, with 1% Ultrapure Bovine serum albumin, Ambion, and 0.2U/μl RNase inhibitor). .. Isolated nuclei were filtered through a 30μm cell strainer (MACS SmartStrainers), washed with 1ml of wash buffer, centrifuged (500g, 5 min, 4°C), resuspended in 300μl of wash buffer on ice, counted using Trypan blue (ThermoFisher) and stained with primary antibodies, including human nuclear antigen conjugated to Phycoerythrin (HNA-PE, 1:100, Abcam) and rabbit anti neuronal nuclei (rb-NeuN, 1:100, Abcam).

    Article Title: Scalable synthetic peptide hydrogel enables self-organized luminal cavity architecture within hiPSC 3D colonies supporting functional hepatocyte differentiation
    Article Snippet: PG-hiHs were retrieved and fixed using 10% neutral buffered formalin (NBF) (Sigma). .. Following fixation, the 3D colonies were rinsed with wash buffer (DPBS with Ca 2+ /Mg 2+ supplemented with 0.2% Triton-X 100 and 0.1% gelatin from cold water (all from Sigma‒Aldrich) and subsequently incubated in 10% normal goat serum (Thermo Fisher) in wash buffer overnight for blocking. ..

    Article Title: Protein-state dysregulation and sex-specific neurodevelopmental signatures in schizophrenia forebrain organoids
    Article Snippet: .. After primary antibody incubation, cultures were washed three times in wash buffer (PBS/0.1% Triton X-100) for 15 min at RT and incubated with appropriate fluorophore-conjugated secondary antibodies and nuclei counterstained with DAPI (NucBlue, #R37606, Thermo Fischer Scientific, 1:1000) diluted in blocking buffer, ON at 4°C. ..

    Article Title: Trp53 loss drives the neoplastic transformation of Pik3ca H1047R -induced vascular malformation in a mouse model
    Article Snippet: .. After washing three times with wash buffer (0.1% Triton X-100 in DPBS, 10 min each), samples were incubated with DAPI for 1 min and mounted in ProLong TM Gold Antifade Mountant (Invitrogen, P10144 ). .. Images were acquired with a Leica SP8 confocal microscope using a 63x oil-immersion lens with a 1 μm optical section.

    Article Title: ZNF184 negatively regulates HR repair and predicts poor prognosis in acute lymphoblastic leukemia.
    Article Snippet: .. Slides and cells were washed three times with wash buffer (PBS containing 0.1% TritonX-100) and subsequently incubated with secondary antibodies conjugated with fluorophores (Alexa Fluor 594 or Alexa Fluor 488) and 4,6-diamidino-2-phenylindole (DAPI, 1 μg/ml, Thermo Fisher Scientific, D1306) for 1 h at RT. .. After three washes with wash buffer, slides were mounted using VectaMount AQ Aqueous Mounting Medium (Vector Laboratories, H-5501).

    Article Title: Optogenetic cochlear stimulation evokes midbrain activity with near-physiological temporal fidelity
    Article Snippet: Samples were blocked with goat serum dilution buffer (16% goat serum, 450 mM NaCl, 0.6% Triton X_100, 20 mM phosphate buffer, pH 7.4) for 2 h at room temperature, then incubated in primary antibodies in goat serum dilution buffer (chicken anti-GFP, 1:500, #ab13970, Abcam, USA, guinea pig anti-parvalbumin polyclonal (Cat No. 195004) 1:200 or monoclonal (Cat No. 195008), Synaptic Systems, Germany) at 4°C for 3 days. .. Next, samples were washed in wash buffer (20 mM phosphate buffer, 0.3% Triton X-100, 0.45 M NaCl) 3x for 10 min and incubated in the secondary antibodies in goat serum dilution buffer (goat anti- chicken 488, 1:200, A11039, Life Technologies- Invitrogen and goat anti-guinea-pig 568, 1:200, A11075, Life Technologies- Invitrogen) for 2 to 3 days at 4°C in the dark. ..

    Article Title: Trp53 loss drives the neoplastic transformation of Pik3caH1047R-induced vascular malformation in a mouse model.
    Article Snippet: .. After washing three times with wash buffer (0.1% Triton X-100 in DPBS, 10 min each), samples were incubated with DAPI for 1 min and mounted in ProLongTM Gold Antifade Mountant (Invitrogen, P10144). .. Images were acquired with a Leica SP8 confocal microscope using a 63x oil-immersion lens with a 1 μm optical section.



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